In Table 1, which bacteria are included in the “++” growth in section “cI+cII” section of plate IV?

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The question asks which bacteria are included in the “++” growth in the section labeled “cI+cII” on plate IV, as presented in Table 1.

Option 1: “cI and conjugates.” This would imply that both bacteria carrying the cI element and bacteria capable of conjugation contribute to the ++ growth. However, if the assay is designed to distinguish conjugation events separately from bacteria that only carry a cI element, the presence of cI alone would typically yield a different growth category (e.g., + or -) and not necessarily the same “++” result, making this option less likely.

Option 2: “conjugates only.” If the ++ growth specifically indicates successful conjugation events or the phenotype associated with conjugates, then only conjugating bacteria would show this ++ category, and pure cI carriers would not. This aligns with a setup where conjugation status drives the strongest (++), distinct from non-conjugating cI carriers.

Option 3: “cI and cII.” This pairing suggests that both cI and cII carriers contribute jointly to the ++ growth. Unless the assay is designed to require both cI and cII presence simultaneously to yield the highest growth class, this combination would generally not be the exclusive cause of ++ growth in a cI+cII section.

Option 4: “cI and cII and conjugates.” This option posits that all three groups contribute to the same ++ category. If the experiment separates phenotypes by individual genetic elements and by conjugation status, grouping all three into a single ++ class would blur distinct sources of the signal and is typically unlikely unless the protocol explicitly aggregates them.

Option 5: “cII only.” If the ++ growth is tied to cI-associated effects or to conjugation-driven phenotypes, cII-only carriers would be an unlikely sole source of the strongest growth, unless the assay is specifically designed to map cII effects to ++ growth, which is not implied by the stated section label.

Option 6: “cI and cII.” (Repeated among options) As discussed for Option 3, this implies both chromosomal or plasmid elements are required for ++, which may not match a classification that isolates conjugation-driven performance.

Option 7: “cI only.” If ++ growth is tied to a phenotype that requires the presence of cI alone, this would be the sole source, but the section label “cI+cII” strongly suggests involvement of both cI and cII, making this option unlikely.

Overall, the most consistent interpretation is that the “++” growth in the cI+cII section of plate IV is driven by conjugates, i.e., bacteria capable of conjugation. This interpretation distinguishes conjugating entities from those merely carrying cI or cII elements, and aligns with a readout where conjugation activity yields the strongest growth signal, separate from non-conjugating strains. Therefore, the option that identifies conjugates as the sole contributors to the “++” growth best fits a typical experimental design that uses a distinct ++ category for conjugative activity.

W25 BIOL D006B 01Y Cell & Molecular Biology Lab Quiz 2

In Table 1, which bacteria are included in the “++” growth in section “cI+cII” section of plate IV?

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