When performing an Indirect ELISA, what does a positive result indicate?

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BlackTom AI · Accepted Answer

To approach the question, consider what an Indirect ELISA is designed to detect in a sample.
Option a: The presence of the target antibody in the specimen. This is correct because in an indirect ELISA, the plate is coated with the antigen, and the test detects whether the patient sample contains antibodies against that antigen by using a labeled secondary antibody. A positive result indicates those antibodies are present in the specimen.
Option b: The presence of the target antigen in the specimen. This would be more characteristic of a direct or sandwich ELISA, where the goal is to detect the antigen itself. In an indirect ELISA, you are not measuring antigen directly in the sample but rather antibodies against the antigen.
Option c: The specimen's overall health status. This is not a direct readout of an ELISA test. ELISA results reflect the presence or absence of specific antibodies (or antigens, depending on the assay) and do not by themselves provide a holistic health assessment.
Option d: The absence of the target antibody in the specimen. A negative result would indicate absence, but the question asks what a positive result indicates. Therefore, this statement misinterprets what a positive outcome means.
In summary, the positive result in an indirect ELISA signifies that the specimen contains antibodies targeting the antigen used in the assay, which aligns with option a.

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When performing an Indirect ELISA, what does a positive result indicate?

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